The cancer cell (Ramos cells) can be detected by the Polymerase Chain Reaction technique developed by freeing the Electrochemiluminiscence. This strategy is based on the ECL nanoprobes and aptamers which contained gold nanoparticles (AuNPs),linker DNA and TBR labeled signal DNA (tris-(2,2′-bipyridyl) ruthenium). The AuS bonds modify the signal DNA and linker DNA which are present on the surface of the AuNPs. The magnetic bicomplex is formed by the aptamers loaded on the magnetic beads which are hybridized by the linker DNA. The cancer cell will be conjugated with the hydridized aptamers with higher affinity and releases the ECL nanoprobe from the biocomplexwhich gets hybridized on the Au electrode along with the modified captured DNA.
The amount of the cancer cell willbe calculated directly from the ECL intensity which is loaded with TBR on the nanoprobes. The detection can be limited as low as 50 Ramos cells/ml can be detected with this developed electrochemiluminescence probe. There are wide applications of these ECL based methods in detecting cancerous cells because of the low cost, simplicity and high sensitivity
The post Cancer Cell Detection by an Electrochemiluminescence appeared first on Science Blog.